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International Journal of Forensic Medicine

2024, Vol. 6, Issue 2, Part A

Methanol fraction of ethanol extract of Dialium guineense stem bark reduces oxidative stress in STZ-induced diabetic rat pancreas


Author(s): Abu OD, Ojo I, Awhin EP and Iyorah I

Abstract: The present study investigated the effect of methanol fraction of ethanol extract of Dialium guineense (MEDG) stem bark on streptozotocin (STZ)-induced oxidative stress in rat pancreas. Adult male Wistar albino rats (n = 25, mean weight = 215 ± 15 g) were assigned randomly to five groups (5 rats per group): normal control, diabetic control, metformin, MEDG (200 mg/kg body weight, bwt) and MEDG (300 mg/kg bwt) groups. A single intraperitoneal injection of 50 mg/kg bwt STZ was used to induce diabetes mellitus in the experimental rats. Treatment with the extract or standard antidiabetic drug (metformin) lasted 21 days. Activities of catalase, superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GR) as well as glutathione (GSH), total protein (TP), malondialdehyde (MDA) and nitric oxide (NO) levels were measured in rat pancreas. The results obtained indicated that induction of diabetes mellitus with STZ significantly increased the fasting blood glucose (FBG) levels of the rats, but it decreased the activity/concentration of antioxidants (enzymes/molecules) (p< 0.05). However, treatment of the diabetic Wistar albino rats with MEDG (200 and 300 mg/kg bwt, respectively) markedly reduced the FBG level and body weights of rats, but enhanced the activity/concentration of antioxidant enzymes/molecules in pancreatic tissue (p< 0.05). The findings from this study have shown that MEDG stem bark has the capacity to enhance antioxidant defense in pancreases of STZ-induced diabetic rats.

DOI: 10.33545/27074447.2024.v6.i2a.89

Pages: 12-19 | Views: 209 | Downloads: 76

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International Journal of Forensic Medicine
How to cite this article:
Abu OD, Ojo I, Awhin EP, Iyorah I. Methanol fraction of ethanol extract of Dialium guineense stem bark reduces oxidative stress in STZ-induced diabetic rat pancreas. Int J Forensic Med 2024;6(2):12-19. DOI: 10.33545/27074447.2024.v6.i2a.89
International Journal of Forensic Medicine

International Journal of Forensic Medicine

International Journal of Forensic Medicine
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